Opto Edu A50.7098 Plunge Freezing Sample Preparation System for Scanning Electron Microscope
The A50.7098 Automated Cryo-TEM Sample Freezing Solution is a grid plunge freezer designed to rapidly fix thin liquid films containing samples into vitrified ice layers using cryogens such as liquid ethane. This system prepares proteins, cells, and solution system materials for subsequent 3D Cryo-TEM analysis.
This equipment meets the sample preparation needs of various types of samples including proteins, large particle suspensions, and viruses.
| NO. |
Parameter |
Specification |
| 1 |
Humidity Control |
Maximum humidity can reach 100% and remain stable. Capable of real-time monitoring of sample chamber humidity, with ultrasonic control function and control accuracy of ±1%. |
| 2 |
Temperature Control |
When ambient temperature is between 18°C and 25°C, chamber temperature can be controlled within 4°C to 60°C, adjustable in 0.5°C increments. |
| 3 |
Sample Injection |
Capable of preparing small-volume samples of 2-10 ul using pipette for manual sample loading. |
| 4 |
Sample Adsorption Method |
Double-sided adsorption. |
| 5 |
Sample Blotting Method |
Blotting using filter paper. Blotting action and vitrification time are controllable. Users can customize blotting compensation and draining time via software. |
| 6 |
Number of Blotting Cycles per Grid |
≥16 times, with multi-adjustable blotting force level. Both number of times and force can be set via software. |
| 7 |
Sample Loading Method |
Manual touch screen control or foot pedal control. Optional: button control and linkage control. |
| 8 |
Plunging Depth |
Adjustable from 1 to 5 levels. |
| 9 |
Blotting Control |
All controllable via software; single blotting time is independently adjustable. Number of blotting actions is adjustable. |
| 10 |
Application Time |
Application time and waiting time can be controlled via software, with relevant parameters pre-settable. |
| 11 |
Secondary Coolant Reservoir Capacity |
Dedicated container with <10 mL capacity. |
| 12 |
Grid tweezers descent speed |
<1 second. |
| 13 |
Temperature control of coolant container and cryo-sample holder |
Capable of synchronously lowering temperature while keeping grid submerged in coolant. Coolant container can include integrated anti-contamination ring. |
| 14 |
Method for transferring grids from coolant to grid box in liquid nitrogen environment |
Semi-automatic. Capable of transferring 4 or more grid boxes at one time. |
| 15 |
Chamber lighting |
Chamber equipped with lighting for easy observation and operation; can be turned off. |
| 16 |
Dewar Cup |
Dewar cup can be moved upward and can be used to pour out excess liquid nitrogen. |
| 17 |
Shutter Operation |
Equipped with automatic shutter control function. |
| 18 |
Gripper Config |
Equipped with motor-driven gripper to ensure highly repeatable clamping force. |
| 19 |
Power Supply |
220V power supply, 300W power. |
